association between efficient adhesion to hep-2 cells and severity of gastritis in helicobacter pylori strains isolated from children
نویسندگان
چکیده
background and objectives: helicobacter pylori causes chronic gastritis, peptic ulceration, and is a risk factor for gastric cancer. more than half of the world`s population is infected with this pathogen, however the disease outcome varies. among the virulence factors of h. pylori, specific adhesions to gastric epithelium may be an important step in the induction of active inflammation. h. pylori displays considerable genetic diversity attributed to genetic drift during long-term colonization. heterogeneity of clinical-isolates has considerably impacted in understanding the role of each factor in infection outcome. however, genetic change may be more limited in the stomach of children in the short period after infection. this work evaluates differences in adherence potential of strains isolated from children with various chronic- inflammation status. materials and methods: a total of 157 children admitted to medical center of tehran for upper gastrointestinal problems underwent endoscopy for h. pylori infection. histological examination of their biopsies was performed after h& e and giemsa staining. gastritis and inflammation were graded according to the updated sydney system. 70 culture-positive children, 20 were selected according to their histopathological status. adherence of their h. pylori isolates to hep- 2 cells was evaluated by viable count of bacteria associated with host cells in an optimized procedure developed in this study. results: correlation was seen between inflammation severity score and efficiency of adhesion to hep-2 cells. this correlation was not observed in the kb cell line as an epithelial cell-model. conclusion: specific interaction of h. pylori with host gastric epithelium may be associated with disease outcome
منابع مشابه
Association between efficient adhesion to HEp-2 cells and severity of gastritis in Helicobacter pylori strains isolated from children
Background and objectives: Helicobacter pylori causes chronic gastritis, peptic ulceration, and is a risk factor for gastric cancer. More than half of the world`s population is infected with this pathogen, however the disease outcome varies. Among the virulence factors of H. pylori, specific adhesions to gastric epithelium may be an important step in the induction of active inflammation. H. pyl...
متن کاملTHE ASSOCIATION OF CAMPYLOBACTER PYLORI (HELICOBACTER PYLORI) WITH GASTRITIS AND PEPTIC ULCER
We studied the relationship between gastroduodenal inflammation and the presence of Campylobaeter pylori from biopsy specimens of the gastric mucosa in 91 patients with gastritis with or without ulcers and 9 controls, healthy or suffering from other diseases. 68 of the patients were positive for the presence of C. pylori in their gastric mucosa. C. pylori was confirmed bacteriohistological...
متن کاملAdherence patterns of diarrheagenic Escherichia coli strains isolated from children with gastrointestinal diarrhea and matched controls in a Hep-2 cell adhesion assay
In the present study, carbon species including organic carbon (OC), elemental carbon (EC), and water-soluble organic carbon (WSOC) concentration in PM2.5 were assessed at an urban site of Tehran, Iran during March to June 2014. The PM2.5 samples were collected using an frmOMNITM Ambient Air Sampler. Thermal gravimetric analysis (TGA) was used to analyze OC and EC. The results showed that PM2.5 ...
متن کاملSensitivity to nitazoxanide among metronidazole resistant Helicobacter pylori strains in patients with gastritis
Background: In this study, the efficacy of nitazoxanide in the treatment of Helicobacter pylori isolates, which were resistant to metronidazole, was examined. Methods: One hundred twenty two patients who underwent endoscopy examinations at Kasra and Laleh hospitals in Tehran from November 2014 to July 2015 were enrolled. Helicobacter pylori strains were isolated from the patients&rsqu...
متن کاملبررسی چسبندگی هلیکوباکترپیلوری به هفت رده سلول کشت شده انسانی
Background and Aim: Helicobacter pylori is the etiologic agent of chronic –active gastritis, gastroduodenal ulcers in humans, and a co-factor in the occurrence of gastric cancer and mucosa-associated lymphoid tumors, Adhesion of H.pylori to the gastric mucosa is a critical and also initial step in the pathogenesis of the disease. Bacterial adhesion inhibitory agents provide a novel pharmacologi...
متن کاملPathogenic interactions between Helicobacter pylori adhesion protein HopQ and human cell surface adhesion molecules CEACAMs in gastric epithelial cells
Objective(s): The present paper aims to review the studies describing the interactions between HopQ and CEACAMs along with possible mechanisms responsible for pathogenicity of Helicobacter pylori.Materials and Methods: The literature was searched on “PubMed” using different key words including Helicobacter pylori, CEACAM and gastric.<br ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of microbiologyجلد ۱، شماره ۱، صفحات ۲۳-۳۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023